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Materials and Methods Chemicals and Reagents Mercuric chloride (HgCl 2 , 99.5% purity) was purchased from Sigma-Aldrich Chemical Company India and other required chemicals of analytical grade were procured, Merck India and HIMEDIA Labs, India
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thereafter, two equivalents of GSH are consumed in the two subsequent reductive steps: first a selenenylsulfide intermediate (G) is formed and then the reduced enzyme is regenerated.[ 55 , 114 ] The kinetics of this mechanism were seen to be more complicated than a Michaelis‐Menten process in which an overall kinetic constant can be defined
