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Efficient expression of CTB-FIX in a commercial lettuce cultivar using a cGMP hydroponic system showed stability of lyophilized lettuce at ambient temperatures for years, excision of the antibiotic selection marker, and induction of oral tolerance using a broad dose range, addressing remaining issues regarding cost of production, purification and cold chain/transportation requirements [54, 151]

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doi:10.1242/jcs.126565 44