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However, entry of excessive Ca 2+ into mitochondria leads to mitochondrial Ca 2+ overload, increased ROS production, inhibition of ATP synthesis, opening of mPTPs, and the subsequent release of cytochrome C (Cyto C) and activation of apoptotic pathways (Bernardi, 2+ and ROS further increases mitochondrial membrane permeability, leading to membrane depolarization and mitochondrial swelling
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