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Glyphosate impairs both structure and function of GABAergic synapses in hippocampal neurons

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At the end of the experiment (48 hours), 100 µl were transferred to a cytometer tube, 0.5 µl of 25 µM Calcein-AM were added and, after 15 min of incubation at room temperature, 5 µl of fluorescently labeled beads plus 195 µl of PBS 1X were added and cells were analyzed by flow cytometry