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Isolated epidermal and dermal cells were mixed in a 1:9 ratio and dropped into the upper chamber of transwell cultures, and the lower chamber was filled with 700 μl of Dulbecco’s modified eagle medium/F12 (#MT100 13CV, Corning, USA) medium (#1099-141C, Gibco, USA) containing 10% fetal bovine serum

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Endogenous extraction yielded high quality sulforaphane from broccoli sprouts unveils potent antioxidant and anti-alzheimer's activities