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A volume of 2 µl samples was injected into a Waters XBridge XP BEH Amide column (150 mm length × 2.1 mm i.d., 2.5 µm particle size) maintained at 25 °C, with a Waters XBridge XP VanGuard BEH Amide (5 mm × 2.1 mm i.d., 2.5 µm particle size) guard column