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Since B 12 deficiency results in MMA accumulation before B 12 reduction is seen in the serum [132], methylmalonic acid is a better indicator and preferred marker reflecting B 12 status before B 12 deficiency appears [120]

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We ran Demuxlet with the following parameters: --field GT and --group-list barcodes.txt, where ‘barcodes.txt’ was the cell barcode file generated by Cell Ranger

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