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The sample was then reduced by dithiothreitol and alkylated with iodoacetamide (IAM) before digestion with trypsin

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Meanwhile, certain viruses may employ epigenetic mechanisms—such as neuron-specific microRNAs—to suppress transcription factors, promote viral genome heterochromatinization, and induce glial histone methylation, thereby establishing viral latency and altering synaptic gene expression (195, 196)