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mRNA Analysis mRNA was extracted from pulverized โ80ยฐC pancreas (100 mg), reverse transcribed into complimentary DNA (cDNA), and measured by quantitative PCR analysis for INS (insulin), GCG (glucagon), PPY (pancreatic polypeptide), SST (somatostatin), PDX1 (pancreatic and duodenal homeobox-1), GCK (glucokinase), SLC2A2 [glucose transporter-2 (GLUT-2)], IGF1 (insulin-like growth factor-1), IGF2 (insulin-like growth factor-2), VEGF (vascular endothelial growth factor), HGF (hepatocyte growth factor), MLF2 (myeloid leukemia factor 2), and S15 (ribosomal protein S15) as previously described (LR3, n = 7