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Notably, Pb exposure has been associated with a significant reduction in the number of VGLUT1-positive synaptic elements that co-express Syn, suggesting that although transporter expression per se may remain stable, the synaptic machinery required for effective glutamate release is functionally compromised [102]

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For all practical lab calculations, you can assume the final volume of the solution is equal to the volume of the bacteriostatic water you added