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All reactions were repeated three times and were conducted on Mx3000P (Agilent Technologies, Santa Clara, CA, USA) under the following conditions: denaturation at 95 °C for 10 min, followed by 40 cycles of amplification at 95 °C for 10 s, annealing at 60 °C for 15 s, and strand extension at 72 °C for 15 s
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