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To obtain enough biological material for the experiment, the seven strains were each cultured for three months at 27°C in 100 mL media (175 cm 2 growth flasks, cat: NUN159910, Thermo Fisher Scientific) and then split into 25 cm 2 assay flasks, seeded at 3 x 10 5 cells/mL in a total volume of 31 mL

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Our results showed that during the operation of 114 patients with the Epi-pledget, no systemic cardiovascular problems occurred