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In the first step, cysteine and glutamate are linked in a reaction catalyzed by the γ-glutamylcysteine synthase (γ-GCS) to form γ-glutamylcysteine

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Interestingly, NF-κB p65 and caspase-3 were significantly inhibited by trifluoperazine, a strong calmodulin antagonist, suggesting that the cardioprotective effect conferred by trifluoperazine involves inhibition of NF-κB and apoptosis