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Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure

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2.5 μl of this cell suspension was mixed with 7.5 μl of lysis buffer [200 mM Tris-HCl (pH 7.5), 50 mM EDTA, 0.5% (w/v) SDS] directly on a slide and incubated for 4 min followed by tilting the slides to 30° causing the drops to run down by gravity