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Polymerase chain reaction (PCR) amplification was performed in the presence of 2.25 mM MgCl 2 , 0.08 μM of each primer, 0.2 mM dNTP, 1.25 U of Taq polymerase (Roche Diagnostics, Mannheim, Germany) and 5 μL of each cDNA to a final volume of 50 μL
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The effect of acute exercise on circulating immune cells in newly diagnosed breast cancer patients
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