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The eluate was immediately neutralized with 0.1 M Tris pH 2.0 to achieve a final pH of 8.5 and a final volume of 200 µl

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Later, eleven chalcone derivatives (Figure 13) were synthesized by the Claisen–Schmidt condensation of acetophenones and aromatic aldehydes, or produced with suitable dihydrochalcone and alkyl bromide, or prepared in one-pot synthesis involving acetophenone and aromatic aldehyde under ultrasonication [87], and their anti-inflammatory potential was assessed