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From a mechanistic point of view, metformin can reduce histone acetylation at the CCR8 promoter and inhibit CCR8 expression on tumor-infiltrating Treg cells by upregulating AMPK-activated Sirtuin 2 (SIRT2), thus enhancing the effectiveness of anti-PD-1 immunotherapy (73)
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Prior to donation, a blood sample was drawn as described in Materials and Methods
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