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Moreover, Poisson et al., through a metabolomics analysis, reported that platinum-sensitive and -resistant ovarian cancer cells presented significant differences involving cysteine and methionine metabolism [97]

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Therefore, affinity-purified rEE2 eluates and rMCP insoluble fractions were further subjected to size exclusion chromatography (SEC) purification: For rEE2, suitable eluates were further purified using Superdex 200 increase 10/300 or 16/600 columns (Cytiva, Little Chalfont, UK) on a GE AKTA Explorer system (GE Healthcare)