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mRNA expression of Gsta4 was highest in the stomach, while lower levels of Gsta4 mRNA were observed in the liver and kidney

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Adding lysate into the culture dish.After lysis, the cells were scraped with one clean scraper on one side of the dish, and the cell debris and lysate were transferred to a 1.5 mL EP tube and centrifuged at 12,000 g for 15 min at 4 ℃