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For the other 22 amino acids, the calibration standards were 0.1 (LLOQ), 0.2, 0.5, 2.0, 10, 40, 80, and 100 µg/mL (ULOQ), with QC samples at 0.3 (LQC), 6 (MQC), and 60 µg/mL (HQC)