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found that acetaminophen causes concentration-dependent neuronal death in vitro at concentrations that are reached in human plasma during acetaminophen overdose, and are reached in the cerebrospinal fluid of rats for 3 h following doses that are below those required to induce acute hepatic failure in rats

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ImageJ software (ImageJ 1.53s, National Institutes of Health, Bethesda, MD, USA) was used to quantify the percentage of CaOx crystal deposition area in each kidney