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Concentration (mcg/mL) = Total peptide in vial (mcg) ÷ BAC water added (mL) Dose volume (mL) = Target dose (mcg) ÷ Concentration (mcg/mL) Syringe units = Dose volume (mL) × 100 (for U-100 insulin syringes) Example: 10 mg BPC-157 vial + 2 mL bacteriostatic water → 10,000 mcg ÷ 2 mL = 5,000 mcg/mL concentration → For a 500 mcg dose: 500 ÷ 5,000 = 0.10 mL → 0.10 mL × 100 = 10 units on a U-100 syringe Reconstitution Verification Checklist Vial peptide content confirmed (check label — mg, not mcg) BAC water volume matches the target concentration for the protocol being referenced

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