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The click reaction is halted with 5 mM EDTA (HY-Y0682), and proteins are precipitated by adding 200 μL water, 400 μL methanol and 100 μL chloroform followed by centrifuging at 6,000 × g for 5 min
Brain Function
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HIV-1/cocaine induced oxidative stress disrupts tight junction protein-1 in human pulmonary microvascular endothelial cells: role of Ras/ERK1/2 pathway
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