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The qPCR cycling conditions were as follows: an initial denaturation at 95 °C for 15 min, followed by 30 cycles of 95 °C for 7 s and 58 °C for 10 s, and a final extension step at 95 °C for 5 min, then 40 cycles of 95 °C for 15 s and 58 °C for 30 s