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If they didn't budge, consider whether the dose was sufficient, the duration was long enough, or whether BPC-157 was the right tool for your specific issue

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Protein samples were mixed with 5× protein loading buffer to a final concentration of 1× and then boiled at 95 °C for 10 min, resolved on 12% Bis–Tris SDS–PAGE gels and analyzed using a standard immunoblotting protocol