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Guide and passenger RNAs were each chemically synthesized (IDT) and gel-purified on a urea-polyacrylamide gel, then annealed into duplexes at 5 µM final concentration in annealing buffer (30 mM Tris-HCl pH 7.5, 100 mM NaCl and 1 mM EDTA)
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Scent:Fig & Amber
doi: 10.7326/0003-4819-118-8-199304150-00009
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