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At the final step after the third KPBS wash, the entire bead volume was lysed in 70□μl of lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100 with protease inhibitors (Roche)

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In vitro , butyrate and propionate also suppress LPS-induced maturation of human monocyte-derived dendritic cells, leading to reduced IL-12 secretion and diminished CD8 + T cell activation (Nastasi et al., 2017)