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Lysosomes are single membrane-enclosed vesicles with a pH of 4.5-5.0 that are made up of 7–10 nm phospholipid bilayers, containing unique acidic lumens (139, 140)
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Placental tissue samples previously stored in RNAlater® at −80°C were allowed to thaw on ice, and were subsequently used for total RNA extraction and relative gene expression quantification using reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
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