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Flow Cytometry Analysis Cells were blocked with anti-mouse CD16/32 Antibody (Biolegend, San Diego, CA) at room temperature for 10 minutes and incubated with fluorochrome-conjugated primary antibody at 4 °C for 30 minutes
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The fine-tuning of TRAF2-GSTP1-1 interaction: effect of ligand binding and in situ detection of the complex
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