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The 4 th round phage output pool was cloned into the yeast display vector pYD1 (Creative Biogene, OVT2893) in scFv format and subjected to two rounds of positive sorting on a Melody FACS (BD, FACSChorus 3.0)

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In mainly Archaea or amitochondrial eukaryotes, GSH is not generally found, leading Copley and Dhillon to suggest that bacteria transferred the genes for GSH biosynthesis to eukaryotes via the progenitor of mitochondria [4]