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In their study, a combined method based on random mutation and rational design improved the activity and catalytic efficiency of key enzymes for ERG synthesis, significantly enhancing ERG yield and achieving high-efficiency production in a short time

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Prior to mitochondrial isolation, 50-200 μL of Pierce Anti-HA magnetic beads (VWR, PI88837) were washed in KPBS (136 mM KCl [Sigma-Aldrich, P5405], 10 mM KH 2 PO 4 , pH 7.4 in HPLC grade H 2 O [Fisher Chemical, W5-1]) for each sample