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Step 1: Calculate Total Bacteriostatic Water Volume Required Use this formula to determine how much bacteriostatic water (BAC) produces your target concentration: (Total peptide mass in mcg) ÷ (Desired concentration in mcg/mL) = Total BAC water in mL Example 1: You have a 5mg kisspeptin vial and want a final concentration of 1000mcg/mL (which delivers 100mcg per 0.1mL injection)