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In intrahepatic cholangiocarcinoma (ICC), distinct gut microbiota compositions, BA metabolic profiles (e.g., altered primary and secondary BAs), and associated cytokine patterns have been identified, which could aid in differentiating ICC from other liver conditions and predicting prognosis (169)
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The combined PQ and FA treatment was performed in the same way as the NAC/FA treatment, with the exception that PQ was added at the same time as FA (2 mmol L −1 ) and incubated together for a total of 5 h