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Research endpoints in optic nerve crush or demyelination models: pattern electroretinography (PERG P50 amplitude, reflecting inner retinal/RGC function), flash visual evoked potentials (FVEP P1 latency, reflecting optic nerve conduction), optic nerve cross-section axon density (toluidine blue semi-thin sections), MBP/MAG myelin immunostaining, and retrograde RGC labelling (Fluorogold intracollicular injection prior to optic nerve injury to mark surviving RGC projections)
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