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For this purpose, PCR primers were designed as follows, where a polyhistidine tag (His-Tag) was incorporated in the reverse primer: VEGF-F: 5′-ACATCAGGTACCATGAACTTTCTGCTGTCTTGG -3′, and VEGF-R: 5′-ACATCTGGTACCAGCGGCCGCATGATGATGATGATGATGCCGCCTCGG CTTGTCAC -3′
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